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Enhanced FGFR3 activation in vemurafenib-resistant B-RAF V600E melanoma cells. A, phospho-RTK antibody array analysis. Cell lysates from A375, M14, A375-R1, and M14-R cell lines were incubated on RTK antibody array for 16 h and phosphorylation status was determined as described under “Experimental Procedures.” Each RTK antibody is spotted in duplicate. Supplemental Table S2 describes the list of RTKs and the layout of the antibody array. B, confirmation of phospho-FGFR3 levels by Western blot analysis. Protein levels of total and phosho-FGFR3 were assessed using immunoblotting. C, <t>ELISA</t> analysis of secreted <t>FGF2</t> in the conditioned media obtained from A375, A375-R1, M14, and M14-R cells. ELISA was performed as described in “Experimental Procedures.”
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Enhanced FGFR3 activation in vemurafenib-resistant B-RAF V600E melanoma cells. A, phospho-RTK antibody array analysis. Cell lysates from A375, M14, A375-R1, and M14-R cell lines were incubated on RTK antibody array for 16 h and phosphorylation status was determined as described under “Experimental Procedures.” Each RTK antibody is spotted in duplicate. Supplemental Table S2 describes the list of RTKs and the layout of the antibody array. B, confirmation of phospho-FGFR3 levels by Western blot analysis. Protein levels of total and phosho-FGFR3 were assessed using immunoblotting. C, <t>ELISA</t> analysis of secreted <t>FGF2</t> in the conditioned media obtained from A375, A375-R1, M14, and M14-R cells. ELISA was performed as described in “Experimental Procedures.”
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quantikine hs human fgf basic immunoassay kit  (R&D Systems)
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Enhanced FGFR3 activation in vemurafenib-resistant B-RAF V600E melanoma cells. A, phospho-RTK antibody array analysis. Cell lysates from A375, M14, A375-R1, and M14-R cell lines were incubated on RTK antibody array for 16 h and phosphorylation status was determined as described under “Experimental Procedures.” Each RTK antibody is spotted in duplicate. Supplemental Table S2 describes the list of RTKs and the layout of the antibody array. B, confirmation of phospho-FGFR3 levels by Western blot analysis. Protein levels of total and phosho-FGFR3 were assessed using immunoblotting. C, <t>ELISA</t> analysis of secreted <t>FGF2</t> in the conditioned media obtained from A375, A375-R1, M14, and M14-R cells. ELISA was performed as described in “Experimental Procedures.”
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Clinical characteristics of individuals with undetectable and detectable <t> serum FGF1 levels. </t>
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elisa assay  (R&D Systems)
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Clinical characteristics of individuals with undetectable and detectable <t> serum FGF1 levels. </t>
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enzyme linked immunosorbent assays  (R&D Systems)
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Clinical characteristics of individuals with undetectable and detectable <t> serum FGF1 levels. </t>
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Clinical characteristics of individuals with undetectable and detectable <t> serum FGF1 levels. </t>
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Image Search Results


Enhanced FGFR3 activation in vemurafenib-resistant B-RAF V600E melanoma cells. A, phospho-RTK antibody array analysis. Cell lysates from A375, M14, A375-R1, and M14-R cell lines were incubated on RTK antibody array for 16 h and phosphorylation status was determined as described under “Experimental Procedures.” Each RTK antibody is spotted in duplicate. Supplemental Table S2 describes the list of RTKs and the layout of the antibody array. B, confirmation of phospho-FGFR3 levels by Western blot analysis. Protein levels of total and phosho-FGFR3 were assessed using immunoblotting. C, ELISA analysis of secreted FGF2 in the conditioned media obtained from A375, A375-R1, M14, and M14-R cells. ELISA was performed as described in “Experimental Procedures.”

Journal: The Journal of Biological Chemistry

Article Title: Reactivation of Mitogen-activated Protein Kinase (MAPK) Pathway by FGF Receptor 3 (FGFR3)/Ras Mediates Resistance to Vemurafenib in Human B-RAF V600E Mutant Melanoma *

doi: 10.1074/jbc.M112.377218

Figure Lengend Snippet: Enhanced FGFR3 activation in vemurafenib-resistant B-RAF V600E melanoma cells. A, phospho-RTK antibody array analysis. Cell lysates from A375, M14, A375-R1, and M14-R cell lines were incubated on RTK antibody array for 16 h and phosphorylation status was determined as described under “Experimental Procedures.” Each RTK antibody is spotted in duplicate. Supplemental Table S2 describes the list of RTKs and the layout of the antibody array. B, confirmation of phospho-FGFR3 levels by Western blot analysis. Protein levels of total and phosho-FGFR3 were assessed using immunoblotting. C, ELISA analysis of secreted FGF2 in the conditioned media obtained from A375, A375-R1, M14, and M14-R cells. ELISA was performed as described in “Experimental Procedures.”

Article Snippet: Cells were cultured for 48 h in growth medium described above, and the conditioned medium samples (cell free culture supernatant) were analyzed for concentrations of human FGF2 using human FGF2 Quantikine ELISA Kit (R&D Systems).

Techniques: Activation Assay, Ab Array, Incubation, Phospho-proteomics, Western Blot, Enzyme-linked Immunosorbent Assay

Clinical characteristics of individuals with undetectable and detectable serum FGF1 levels.

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Clinical characteristics of individuals with undetectable and detectable serum FGF1 levels.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Metabolic parameters of individuals with undetectable (group 1), low (group 2), and high (group 3) serum FGF1 levels.

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Metabolic parameters of individuals with undetectable (group 1), low (group 2), and high (group 3) serum FGF1 levels.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Metabolic parameters of individuals with undetectable (group 1), low (group 2), and high (group 3) serum FGF1 levels. DI, disposition index; IGI, insulinogenic index; ISI, Matsuda insulin sensitivity index.

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Metabolic parameters of individuals with undetectable (group 1), low (group 2), and high (group 3) serum FGF1 levels. DI, disposition index; IGI, insulinogenic index; ISI, Matsuda insulin sensitivity index.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Univariable and multivariable analysis of the association between serum FGF1 levels and metabolic parameters using a Tobit regression model.

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Univariable and multivariable analysis of the association between serum FGF1 levels and metabolic parameters using a Tobit regression model.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Box plot of the estimated log-transformed serum FGF1 level according to the quartile of each parameter using a Tobit regression model adjusted by age and sex. DI, disposition index; IGI, insulinogenic index; ISI, Matsuda insulin sensitivity index.

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Box plot of the estimated log-transformed serum FGF1 level according to the quartile of each parameter using a Tobit regression model adjusted by age and sex. DI, disposition index; IGI, insulinogenic index; ISI, Matsuda insulin sensitivity index.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques: Transformation Assay